Gas chromatography (GC) and High Performance Liquid Chromatography (HPLC)

Chromatography principles and theoretical development.

Partition, stationary and mobile phases, types of interactions between compounds with stationary phase, chromatogram, retention time, adjusted retention time, relative retention, capacity factor, resolution, response factor, effect of diffusion, standard deviation of peak width, theoretical plates, band spreading, plate height, van Deemter equation, type of GC columns, stationary phases for GC, packed GC columns, carrier gas, retention index, temperature and pressure programming, sample injection, injection ports, detectors.Stationary phase for HPLC, solvents, solvent pumps and mechanism, sample injection, valves, solvent programming, detectors.